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Ussing chamber study Nonfasted mice were dissected under isoflurane anesthesia, and the cecal tissue was removed and washed well with Krebs Ringer solution (117 mM NaCl, 5.9 mM KCl, 2.5 mM CaCl 2 , 1.2 mM MgCl 2 , 24.8 mM NaHCO 3 , 1.2 mM NaH 2 PO 4 , and 11.1 mM glucose)

Key quality attributes: Purity: 99% for each component peptide, COAverified by HPLC and complementary analytical methods

The loading phase is extended, with injections given on alternate days until no further improvement is observed, which may take several weeks or months

However, the authors concluded that the higher dose of HFC combined with MSM was crucial for the improvement in skin hydration

doi:10.1055/s-0034-1394133

However, this indicator may be influenced by other factors, such as low vitamin B6 or folate levels